Journal: Journal of Toxicologic Pathology

Article Title: Evaluation of the teratogenic effects of three traditional Chinese medicines, Si Jun Zi Tang, Liu Jun Zi Tang and Shenling Baizhu San, during zebrafish pronephros development

doi: 10.1293/tox.2013-0045

Figure Lengend Snippet: CMP-induced kidney malformation phenotypes are dose dependent. Morphological changes of Tg( wt1b :GFP) embryos after exposure to 25, 250, and 1,250 ng/mL of CMP (SJZT, LJZT, SLBS). Phenotype percentages of zebrafish embryos after CMP treatments using exposure methods I, II, and III (A, B, C). All photos were taken from a dorsal view at the developmental stage of 48 hpf. (D) The Tukey-Kramer HSD (honestly significant difference) test reported the marginal mean malformation rates and corresponding 95% confidence intervals for combinations of the method and CMP groups, which were adjusted for dose effect. Two group means are significantly different if their intervals are disjoint; and are not significantly different if their intervals overlap. An arrow, star and triangle indicate the defective sites in gl, pt; and pd, respectively. Scale bar: 40 μm.

Article Snippet: All embryos were observed under a microscope (DM 2500, Leica) equipped with Nomarski differential interference contrast optics and a fluorescent module having a GFP filter cube (Kramer Scientific).

Techniques:

Journal: Frontiers in Microbiology

Article Title: Enabling oxygen-controlled microfluidic cultures for spatiotemporal microbial single-cell analysis

doi: 10.3389/fmicb.2023.1198170

Figure Lengend Snippet: Overview of the microfluidic cultivation system for spatiotemporal microbial single-cell analysis under a controlled O 2 environments. (A) O 2 control was achieved by using a microfluidic chip with (de)oxygenator structure inside an 3D-printed mini-incubator over the range of 0% - 100%. (B) O 2 sensing with spatial and temporal resolution was implemented by imaging the fluorescence lifetime of the O 2 -sensitive dye RTDP. (C) Automated image acquisition and analysis was performed with special software tools.

Article Snippet: The fluorescence excitation light was supplied by a SOLA LED (SOLA Light Engine, Lumencor, USA) and a fluorescence filter cube (GFP-3035D, Nikon, Japan).

Techniques: Single-cell Analysis, Control, Imaging, Fluorescence, Software

Journal: Frontiers in Microbiology

Article Title: Enabling oxygen-controlled microfluidic cultures for spatiotemporal microbial single-cell analysis

doi: 10.3389/fmicb.2023.1198170

Figure Lengend Snippet: O 2 control performance characterization. (A) Calibration plot of the RTDP sensor solution at 30°C. (B) Imaging ROIs 1 and 2 where FLIM of the RTDP oxygen sensor in the fluid channel was performed (scale bars = 50 μm). (C) Representative FLIM images obtained at defined O 2 gas flow. The images were taken at ROI 1 at 30°C. (D) O 2 concentration over time derived from corresponding fluorescence lifetime images at 30°C at ROI 1 and 2. (E) Final O 2 concentration determined by FLIM over the externally applied gas flow. The dotted line shows the optimum. (F) Switching time analysis: t 90% for each step of the set external O 2 levels. The middle markers indicate t 90% , and the bars indicate t 85% and t 95% .

Article Snippet: The fluorescence excitation light was supplied by a SOLA LED (SOLA Light Engine, Lumencor, USA) and a fluorescence filter cube (GFP-3035D, Nikon, Japan).

Techniques: Control, Imaging, Concentration Assay, Derivative Assay, Fluorescence

Journal: Frontiers in Microbiology

Article Title: Enabling oxygen-controlled microfluidic cultures for spatiotemporal microbial single-cell analysis

doi: 10.3389/fmicb.2023.1198170

Figure Lengend Snippet: Population based analysis using the model organism E. coli MG1655 expressing GFP. (A) Time-lapse image series of a E. coli MG1655 population cultured at 30°C under aerobic conditions and continuous supply of M9 medium supplemented with 2% glucose (00:00 h ≤ t ≤ 09:00 h) (scale bars = 10 μm). (B) Time-lapse image series of E. coli MG1655 population cultured at 30°C under anaerobic conditions and continuous supply of M9 medium supplemented with 2% glucose (00:00 h ≤ t ≤ 08:05 h) with a switch to aerobic conditions (08:10 h ≤ t ≤ 09:00 h) (scale bars = 10 μm). (C) Mean intensity of GFP fluorescence over population ( I population ) and cell number ( N population ) of E. coli MG1655 populations cultured under aerobic conditions. 1875 cells were analyzed at t = 09:00 h. The data are presented as mean ± S.D. denoted by the shaded areas ( n = 5 individual populations). (D) I population and N population of E. coli MG1655 populations cultured under anaerobic conditions with a switch to aerobic conditions. 783 cells were analyzed at t = 09:00 h. The data are presented as mean ± S.D. denoted by the shaded areas ( n = 5 individual populations).

Article Snippet: The fluorescence excitation light was supplied by a SOLA LED (SOLA Light Engine, Lumencor, USA) and a fluorescence filter cube (GFP-3035D, Nikon, Japan).

Techniques: Expressing, Cell Culture, Fluorescence

Journal: Frontiers in Microbiology

Article Title: Enabling oxygen-controlled microfluidic cultures for spatiotemporal microbial single-cell analysis

doi: 10.3389/fmicb.2023.1198170

Figure Lengend Snippet: Single-cell tracking and analysis of fluorescence intensities ( I cell ) and generation numbers. One population was selected for (A–C) aerobic conditions and (D–F) anaerobic conditions with a switch to aerobic conditions. (A, D) Fluorescence intensity images at t = 08:00 h, 08:30 h, and 09:00 h (scale bars = 10 μm). (B, E) Generations number (i.e., number of division events detected) visualized by color coding. (C, F) I cell and generations at three time points.

Article Snippet: The fluorescence excitation light was supplied by a SOLA LED (SOLA Light Engine, Lumencor, USA) and a fluorescence filter cube (GFP-3035D, Nikon, Japan).

Techniques: Single Cell Tracking, Fluorescence